Part:BBa_K988001:Design

K314100_PelB signal sequence_Oxytocin-neurophysin1_6x_His-tag_stop codon

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 470
  Illegal NheI site found at 493
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 126
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We used the PELB signal sequence from E. coli because the Oxytocin protein begins with a cysteine that forms a disulfide bond with the cysteine in position six. This cysteine must be free of any other amino acids. The PELB signal sequence guides the protein to the inner membrane of E. coli and the signal sequence is cleaved as the protein enters the periplasmic space, leaving the position one cysteine free to form disulfide bonds.

Source

This sequence was optimized from the human Oxytocin gene.